GATEWAY VECTOR MANUAL

Gateway Cloning | Thermo Fisher Scientific - US
Invitrogen Gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. From protein expression to functional analysis, Gateway cloning technology is applicable for a
GATEWAY™ Cloning Technology
PDF fileGATEWAY™ Cloning Technology Version 1 Note: This product is covered by a Limited Label License (see Section 1.3) consideration paid for this product grants a Limited License with a paid up royalty to use the product pursuant to the
pBAD/Thio His TOPO manual - Thermo Fisher Scientific
PDF fileFor details about a particular Invitrogen destination vector or expression system, refer to the manual for the specific destination vector or system. All Gateway ® product manuals are available for downloading from our Web site (wwwtrogen) or by contacting Technical Service
Gateway pDONR Vectors - Thermo Fisher Scientific
PDF fileexpression clone into a destination vector without purifying the intermediate entry clone, refer to the Gateway® Technology with Clonase® II manual for a one-tube protocol. Although this protocol allows you to generate expression clones more rapidly than the standard BP reaction followed by the LR reaction, fewer expression clones will be
Plasmids 101: Gateway Cloning
You can use Gateway cloning to insert multiple DNA fragments into many vectors at once in the same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one tube, into one Gateway vector to produce the desired expression clone.
Gateway Destination Vectors | Thermo Fisher Scientific - CA
Destination vector selection guides. Gateway cloning technology is especially noted for its utility in protein expression. The flexibility and diverse selection of Destination vectors and host systems is particularly attractive for multidisciplinary protein expression studies.
Gateway Vector Conversion System
PDF fileInstruction Manual Gateway® Vector Conversion System into a Gateway® destination vector Version B 24 February 2004 25-0527 A Limited Label License covers this product (see Purchaser Notification). By use of this product, you accept the terms and conditions of the Limited Label License. For conversion of any vector of choice Catalog no. 11828-019
Gateway recombination - YouTube
Click to view on Bing9:15This video describes the biochemistry behind the Gateway site-specific recombination system. This video describes the biochemistry behind the Gateway site-specific recombination system. Skip navigation Conditional gene expression using the Cre Lox FLEx vector Author: NCSU BITViews: 19K
GATEWAY™ Cloning Technology
PDF file*Baculovirus Expression Systems provide components to construct a transfer vector. as described in the GATEWAY Cloning Technology Instruction Manual, but does not provide rights to synthesize primers or to perform amplification using primers containing recombination sites or portions thereof.
GATEWAY Binary Vector (pGWB)
PDF file(1) Subsequent to the LR reaction with an Entry Clone and a pGWB vector, the attR1-(CmR, ccdB)-attR2 cassette in the pGWB will be replaced by the attB1-(Your Clone)-attB2. (2)The tag will be fused automatically in-frame to Your Clone, if the primer was designed according to the GATEWAY system (see instruction manual by Invitrogen).